SARS-CoV-2 Identification Using Colorimetric Loop-Mediated Isothermal Amplification
1 Department of Biochemistry, “Victor Babes” University of Medicine and Pharmacy, 300041 Timisoara, Romania; (P.D.M.); (A.I.M.); (I.O.S.)
2 Center for Complex Network Science, “Victor Babes” University of Medicine and Pharmacy, 300041 Timisoara, Romania
3 Department of Pulmonology, “Victor Babes” University of Medicine and Pharmacy, 300041 Timisoara, Romania;
4 Faculty of Medicine, Victor Babes” University of Medicine and Pharmacy, 300041 Timisoara, Romania;
* Correspondence:
* Author to whom correspondence should be addressed.
Received: 21 Dec 2021 / Revised: 25 Jan 2022 / Accepted: 25 Jan 2022 / Published: 16 Feb 2022
Abstract
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused worldwide losses from all points of view. Although there are efficient vaccines against this virus, rapid detection remains essential for controlling its spread. The gold standard for the COVID-19 diagnostic is the use of quantitative real time polymerase chain reaction (RT-qPCR) from naso-/oro-pharyngeal swabs and, alternatively, saliva specimens. A faster alternative to the RT-qPCR method, the colorimetric reverse transcription loop-mediated isothermal amplification (cLAMP) has a good sensitivity and specificity and has been proposed as an efficient screening method. In this study, we analyzed several sets of LAMP primers for COVID-19 testing using purified RNA samples and raw saliva samples, and tested several reaction conditions. Our results showed that the sensitivity of cLAMP reactions on raw saliva samples can be significantly augmented by the addition of guanidine hydrochloride to levels, which allow its use for COVID-19 screening.
Keywords: SARS-CoV-2/COVID-19 testing; saliva specimens; cLAMP
OPEN ACCESS
This is an open access article distributed under the Creative Commons Attribution
License which permits unrestricted use, distribution, and reproduction in any medium,
provided the original work is properly cited. (CC BY 4.0).
CITE
Chiș, A.R.; Muntean, P.D.; Moatăr, A.I.; Oancea, C.; Crețu, O.M.; Sîrbu, I.O. SARS-CoV-2 Identification Using Colorimetric Loop-Mediated Isothermal Amplification. Timisoara_Med 2022, 2022, 1.
Chiș AR, Muntean PD, Moatăr AI, Oancea C, Crețu OM, Sîrbu IO. SARS-CoV-2 Identification Using Colorimetric Loop-Mediated Isothermal Amplification. Timisoara Medical Journal. 2022; 2022(1):1.
Chiș, Aimee Rodica; Muntean, Paula Diana; Moatăr, Alexandra Ioana; Oancea, Cristian; Crețu, Octavian Marius; Sîrbu, Ioan Ovidiu. 2022. "SARS-CoV-2 Identification Using Colorimetric Loop-Mediated Isothermal Amplification." Timisoara_Med 2022, no. 1: 1.
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